HIGH One-Step Acrylamide Solution kit

SDS-PAGE, which is most widely used in electrophoresis for protein separation today, is a method of separating two gels with different pH compositions into discontinuous buffer system.

This method has the advantage of stacking proteins using the potential difference between Cl- and glycine ions generated at pH6.8 even if many samples are loaded. 

However, the disadvantage of a maintaining a high pH level is that the glycine ion must be charged strongly with negative charges in the separating gel to separate proteins. 

In these alkaline states, disulfide bond dissociated by deamination, alkylation or reducing agent(like DTT, β-mercaptoethanol) are reoxidated, affecting protein molecular weight.

One-Step Acrylamide solution is a product that compensates for these shortcomings and minimizes protein changes by maintaining gel and running buffers in an acidic state and inhibits disulfide binding to Cysteine.

Features 

1.     Acid: One-Step Acrylamide Gel pH6.3 & One-Step Running Buffer pH7.0-7.3
-Inhibit protein Deamination, Alkylation & Cystein Oxidation
-Long term Gel storage

2.     Buffer System that maintains a constant loading

3.     Add reducing agent to One-Step Running Buffer to maintain the reducing state

4.     Low cost
-Get One-Step Acrylamide Gel(500ml), 20x One-Step Running Buffer, 200x Reducing agent, APS, TEMED at just 1 bottle of 30% Acrylamide (29:1, 500ml)
-No need to purchase other reagents for Gel electrophoresis

5.     Convenient use and Save time
-Add the kit component APS, TEMED to complete gel
-No need Stacking gel
-Easily separate other size proteins by changing Only the Running Buffer
-1.2 times fast than normal SDS-PAGE

6.     Easily get hard to separate molecular weight in normal SDS-PAGE
-Separate protein above 300kDa
-Separate protein less than 15kDa

Ordering Information