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Chembio® PFUplus DNA Polymerase
The Chembio® PFUplus DNA Polymerase is a highly thermostable DNA polymerase from the hyper-thermophilic archaeum Pyrococcus furiosus. The enzyme catalyzes the template-dependent polymerization of nucleotides into duplex DNA in the 5'→3' direction. Pfu DNA Polymerase also exhibits 3'→5' exonuclease(proofreading) activity that enables the polymerase to correct nucleotide incorporation errors. It has no 5'→3' exonuclease activity.
Good performance of DNA amplification by PCR was confirmed genomic DNA as the template.
: human genomic DNA amplification fragment ~5kb
Overview
• Amplify DNA templates with high sensitivity and high fidelity
• Increase PCR yield and accuracy with PFU DNA polymerase
• Generates Blunt-end PCR products
Ordering Information
Cat. No. | Product | Size |
CBTQ-1220-250 | Chembio® PFUplus Taq DNA polymerase | 250 Unit |
Components | ||
PFUplus Taq DNA polymerase 250 Unit (5U/μl) | ||
10x PFUplus Taq Buffer 800μl | ||
2.5mM dNTPs 500μl | ||
6x Gel Loading Buffer 1ml | ||
CBTQ-1220-500 | Chembio® PFUplus Taq DNA polymerase | 500 Unit |
Components | ||
PRIME Taq DNA polymerase 500 Unit (5U/μl) | ||
10x PFUplus Taq Buffer 1.2ml | ||
2.5mM dNTPs 900μl | ||
6x Gel Loading Buffer 1ml |