Chembio® 1st strand cDNA Kit

Chembio® 1st strand cDNA Kit contains all components necessary for the quantitative conversion of up to 2μg of total RNA to single-stranded cDNA in a single 20μl reaction. 1st strand cDNA Kit delivers extremely high-quality, single-stranded cDNA from 0.02 to 2μg total RNA. The Chembio® Reverse Transcriptase M-MLV is an ultra-pure Molonely Murine Leukemia Virus Reverse Transcriptase designed to virtually eliminate contaminating RNases and other nucleases that are often introduced into the RT reaction with the enzyme. RNase H activity catalase the endonucleaolytic degradation of RNA in RNA/DNA hybrids. The Chembio® Reverse Transcriptase M-MLV is an enzyme which lacks the enzyme is similar to that of wild type enzyme. And the extension ability of this enzyme has been improved remarkably compared to M-MLV (RNase H-) by deleted mutation. Reactions can be scaled up to 100μl to generate 10 μg pf cDNA from a single reaction. Downstream applications include real-time PCR, standard PCR, and Microarray. The Kit is ideal for generating cDNA archives.

Component:

• M-MLV RTase (RNase H-)

• 5x First-Strand Buffer [250mM Tris-HCl pH 83 at Room temperature, 375mM KCl, 50mM DTT, 15mM MgCl_​2]

• RNase Inhibitor

• dNTP Mixture (10mM each)

• Oligo dT Primer

• Random Primer

• RNase Free Water

Applications:

• Synthesis of cDNA 

• Microarray 

• Gene Expression Analysis 

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